MicroRNA 198 Inhibits Invasion and Metastasis in Non-Small Cell Lung Cancer by Targeting Toll-Like Receptor 4

The expression of microRNA 198 which is localized in human chromosome 13q13.3 downregulated many malignant tumors. purpose this study to investigate the regulatory effects on non-small cell lung cancer and associated mechanism. levels lines tumor tissues were determined using reverse transcription polymerase chain reaction method. Overexpression adenocarcinomic alveolar basal epithelial cells, cells proliferation each group measured with counting kit-8 Cell migration was a scratch test, while transwell assay used for determination invasion capacities. Bioinformatics website predict target genes verified double luciferase assay. Western blot method protein level toll-like receptor 4 overexpression regulation by 198. expressions significantly downregulated. However, through transfection inhibited proliferation, directly targeted 4. reversed effect some extent. MicroRNA inhibits targeting 4, thereby playing role suppressor gene development cancer.